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RDS 2012 R2 take long time while opening first application

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Hello experts:

My RDS Environment Have the following servers:

1- Two web access server ( RR DNS )---> virtual machines

2- Two Connection brokers servers (RR DNS) ---> Virtual machines

3- Two host session servers combined as a farm ---> Physical machines with 128 GB of RAM for each server

4- There is no gateway server.

5- The Active Directory is 2008 R2 while the RDS is 2012 R2

My problem is when the users try to access published application they faced a long time ( around 1 min ) to open the first app, the subsequent app open quickly, the problem on when launch the first app.

I tried the following in order to solve the problem with no luck:

- Edit the registry and add DisableTaskOffload with value =1.

- Disable the large send offload v2 IPv4 on all NIC's.

so the problem still exist and i didn't found any solution for it, any help or suggestion will highly appreciated.


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